The Study of Сultural and Secretarial Properties of Hybridomas Producing Monoclonal Antibodies to Ebola Virus during Cultivation on Serum-Free Media

Автор: I.S. Semenova, S.V. Ruchko, I.A. Churkin, V.N. Lebedev, Yu.I. Pashchenko, and A.V. Ovchinnikov

Страница: 25-31

The Study of Сultural and Secretarial Properties of Hybridomas Producing Monoclonal Antibodies to Ebola Virus during Cultivation on Serum-Free Media

Russian Journal of Biotechnology, 2014, N 5, P. 25-31

UDC 6.12.017.1.577.21

Section:  “Producers, Biology, Selection, and Gene Engineering”

I.S. Semenova,  S.V. Ruchko,  I.A. Churkin,  V.N. Lebedev,  Yu.I. Pashchenko ^*,  and  A.V. Ovchinnikov

The 48 Central Scientific Research Institute, Ministry of Defense of RF,  Sergiev Posad, Moskovskaya Oblast Russia

e-mail:  48cnii@mail.ru

The cultural and secretarial characteristics of the E4/N-6G5 and E3/S-8D6 hybridomas producing MCA to the Ebola virus have been studied during their adaptation to a serum-free medium of OptiPRO-SFM or ISF-1, respectively. The obtained MCA titer (1:1280) makes it possible to design diagnostic kits on the basis of these MCA for the virus indication and identification in biological samples.

Key words:  Ebola virus,  hybridomas,  monoclonal antibodies,  serum-free media.

24.03.2015, 1706 просмотров.