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Optimization of Technological Parameters of the Process for D-Tryptophane Producing from Racemate by Microbiological Method
Optimization of Technological Parameters of the Process for D-Tryptophane Producing from Racemate by Microbiological MethodАвтор: A.A. Vardanyan, and A.E. Aghajanyan Страница: 55-62
Optimization of Technological Parameters of the Process for D-Tryptophane Producing from Racemate by Microbiological Method Russian Journal of Biotechnology, 2014, N 4, P. 55-62 UDC 577.112.388.2; 577; 151.52 Section: “Biologicals Technology”
A.A. Vardanyan *, and A.E. Aghajanyan Scientific Production Center Armbiotechnology, Natl. Acad. Sci., Republic of Armenia, 0056, Yerevan Armenia e-mail: and.vardanyan@gmail.com
Obtaining of D-tryptophane from the aminoacid racemate using a strain of Proteus vulgaris AC-16 has been optimized (age of the inoculate, pH, KLa, time and temperature). A stepwise principle of the regulation of the process technological parameters was recommended. As compared to the uncontrolled cultivation, this method provides a higher D-tryptophane yield (97—97.5% of the amount containing in the racemate), reduction of time by 22—25 h and significant decrease in the energy consumption.
Key words: biodegradation, strain-restructor, technological parameters, D-tryptophane, L-tryptophane, tryptophane racemate.
23.03.2015, 1497 просмотров. |
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