Biofilm Formation and Sporulation in Brevibacillus laterosporus

Автор: R.R. Аzizbekyan, L.V. Didenko, T.А. Smirnova, А.I. Кuzin, N.V. Shevlyagina, М.V. Zubasheva, and М.А. Nikolaenko

Страница: 28-34

Biofilm Formation and Sporulation in Brevibacillus laterosporus

Russian Journal of Biotechnology, 2014, N 4, P. 28-34

UDC 616.-093/098:57/087

Section:  “Producers, Biology, Selection, and Gene Engineering”

R.R. Аzizbekyan ^{1, *},  L.V. Didenko ²,  T.А. Smirnova ^{2, *},  А.I. Кuzin ¹,  N.V. Shevlyagina ²,  М.V. Zubasheva ²,  and  М.А. Nikolaenko ¹

¹  The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Моscow Russia

²  The Gamaleya Research Institute for Epidemiology and Microbiology,  123098, Моscow Russia

e-mail:  raziz@genetika.ru,  smiryu@mail.ru

The spore accumulation in B. laterosporus in a liquid nutrient medium under the optimal conditions during stationary cultivation has been investigated. The structure of the floating biofilms was studied using the methods of transmission and scanning electronic microscopy. It was shown that the B. laterosporus cells formed a biofilm during 96 h of incubation. The accumulation of the spores and individual vegetative cells in the biofilms was confirmed by light microscopy. The spore ultrastructure was shown to depend on media, rich or poor. The B. laterosporus demonstrated an unusual morphology when grown on the DPS medium which was shown for the first time. The differences concerning the vegetative cells and spores between B. laterosporus and Bacillus subtilis biofilms were detected. In the Bacillus subtilis biofilm, the vegetative cells are numerous and devoid of defects that are characteristic of B. laterosporus. The obtained results can be helpful for the optimization of the bacilli-based biotechnological processes aimed at improving the yield of target products.

Key words:  Bacillus subtilis,  biofilms,  Brevibacillus laterosporus,  crystals,  scanning microscopy,  spores,  transmission microscopy.

23.03.2015, 1851 просмотр .