Obtaining and Characterization of Antibody-Producing Hybridomas and Monoclonal Immunoglobulins to V. cholerae Enterotoxin

Автор: E.A. Mikheeva, Z.L. Devdariani, N.A. Osina, and T.L.Zakharova

Страница: 49-54

Obtaining and Characterization of Antibody-Producing Hybridomas and Monoclonal Immunoglobulins to V. cholerae Enterotoxin

Russian Journal of Biotechnology, 2014, N 3, P. 49-54

UDC 616.932:612.017.1

Section:  “Biologicals Technology”

E.A. Mikheeva ^*,  Z.L. Devdariani,  N.A. Osina,  and  T.L.Zakharova

The Russian Research Anti-Plague Institute “Microbe”,  410005, Saratov Russia

e-mail:  rusrapi@microbe.ru

A panel of five hybridomas producing monoclonal antibodies to the cholera toxin (CT) has been designed. The obtained monoclonal antibodies (MCA) induced the ascites development in 100% BALB/c mice and were stably produced during in vivo passages. MCA reacted in GM1-solid-phase ELISA with toxigenic epidemically significant V. cholerae strains of both biovariants. The hybrid lines are promising producers of monoclonal immunoglobulin reagents for design and production of immuno-enzyme test systems for the detection of cholera toxin and toxigenic cholera vibrio strains.

Key words:  cholera toxin,  hybridoma technology,  monoclonal antibodies.

19.03.2015, 1568 просмотров.