Human Plasminogen Activation is Blocked by Monoclonal Antibody

Автор: N.M. Rutkevich, T.I. Belyanko, Ya.G. Gurskii, P.G. Sveshnikov, N.A. Skrypina, O.N. Solopova, and R.Sh. Bibilashvili

Страница: 41-48

Human Plasminogen Activation is Blocked by Monoclonal Antibody

Russian Journal of Biotechnology, 2014, N 3, P. 41-48

UDC 577.156.6-576.8.097.3

Section:  “Biologicals Technology”

N.M. Rutkevich ^{1, *},  T.I. Belyanko ¹,  Ya.G. Gurskii ¹,  P.G. Sveshnikov ²,  N.A. Skrypina ¹,  O.N. Solopova ²,  and  R.Sh. Bibilashvili ¹

¹  The Russian Scientific-and-Industrial Complex of Cardiology, Ministry of Health of RF,  121552, Moscow Russia

²  The All-Russian Research Center for Molecular Diagnostics and Therapy,  117149, Moscow Russia

e-mail:  nrutkevich@yandex.ru

An H4 monoclonal antibody that selectively binds to plasminogen (PLG), but not to mini-plasmin has been obtained. This selectivity probably results from the interaction between the antibody and the PLG activation site. The antibody sharply blocks the PLG activation by urokinase-type (uPA) or tissue (tPA) PLG activator. The activation is not blocked by the control G8 antibody which equally binds to PLG and mini-plasmin. The H4 antibody is specific to human PLG; the rabbit PLG activation is not inhibited. The H4 antibody disrupts the cycle of the mutual activation of PLG and uPA: both uPA-dependent PLG activation and PLG-­dependent pro-uPA activation are blocked. Thus, in the presence of the H4 antibody, pro-uPA is not activated by plasmin in a mixture of PLG, pro-uPA and activated uPA, which makes it possible to measure in this mixture of the activated uPA concentration.

Key words:  activator,  monoclonal antibody,  plasmin,  tissue plasminogen activator,  urokinase-type plasminogen activation.

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