Fractionation of Cholerae vibrio Cholerogen-Anatoxin by Tangential Filtration

Автор: A.V. Komissarov, S.A. Eryomin, A.I. Perepelitsa, O.V Gromova, L.F. Livanova, A.V. Gaeva, and A.K. Nikiforov

Страница: 34-40

Fractionation of Cholerae vibrio Cholerogen-Anatoxin by Tangential Filtration

Russian Journal of Biotechnology, 2014, N 3, P. 34-40

UDC 615.371/.372:663.15:66.081.63

Section:  “Biologicals Technology”

A.V. Komissarov ^*,  S.A. Eryomin,  A.I. Perepelitsa,  O.V Gromova,  L.F. Livanova,  A.V. Gaeva,  and  A.K. Nikiforov

The Russian Research Antiplague Institute Microbe,  410005, Saratov Russia

e-mail:  rusrapi@microbe.ru

A method of tangential ultrafiltration was applied for the first time to the production of a cholera bivalent chemical tableted vaccine, namely to the fractionation of the Vibrio cholera 569 Inaba strain cholerogen-anatoxin. The selection of an optimum type of membranes for ultrafiltration was performed. The characteristics of the cholerogen-anatoxin obtained by the developed method were investigated. The use of the method makes it possible to reduce the number of stages in the technological process from 7 to 6 and to decrease the time of the cholerogen-anatoxin isolation from culture liquid and the duration of the whole cycle by 100 h as compared to the existing technology. The losses of the target preparation were also reduced by 25%. The components of the anti-cholera vaccine obtained by the developed method meet the standard requirements.

Key words:  cholera vaccine,  cholerogen-anatoxin,  fractionation,  tangential ultrafiltration.

19.03.2015, 1714 просмотров.