Study of Novel Plant Promoter of Gene proSmAMP2 from Stellaria media by Agrobacterial Infiltration of Plants

Автор: S.R. Strel’nikova, V.D. Voblikova, R.R. Shukurov, A.B. Babakov, and R.A. Komakhin

Страница: 8-17

Study of Novel Plant Promoter of Gene proSmAMP2 from Stellaria media by Agrobacterial Infiltration of Plants

Russian Journal of Biotechnology, 2014, N 3, P. 8-17

UDC 604.6:582, 602.4

Section:  “Producers, Biology, Selection, and Gene Engineering”

S.R. Strel’nikova ^{1, *},  V.D. Voblikova ¹,  R.R. Shukurov ²,  A.B. Babakov ¹,  and  R.A. Komakhin ¹

¹  The All-Russian Research Institute for Agricultural Biotechnology,  127750, Moscow Russia

²  The State Research Institute for Cenetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

e-mail:  strlnkv@yandex.ru

In order to search for a novel plant promoter to be used in plant biotechnology, a nucleotide sequence of the promoter region of gene proSmAMP2 encoding antimicrobial peptides in a weed plant of Stellaria media was sequenced and cloned. To characterize this sequence as a promoter for the expression of genes of interest in heterological plants, two 5'-deletion variants (–2160 and –862 bp) that also included 5'-untranslated region of proSmAMP were produced. These deletion variants were fused with the gus reporter gene sequence in the plant expression vector of pCambia 1381Z and characterized via the transient expression in the cells of Nicotiana benthamiana plants. Evaluations of the GUS activity showed that the –2160 bp deletion in the promoter region of proSmAMP2 makes the GUS activity only slightly different from zero, whereas the –862 bp deletion variant matches in effectiveness to the viral promoter of CaMV35S obviously surpassing the latter in ecological safety. This –862 bp deletion variant of the proSmAMP2 gene promoter region has been suggested as a novel promoter for the transient expression of genes in plants aimed at the rapid synthesis of target proteins.

Key words:  agrobacterium-mediated transient expression of genes,  antimicrobial peptides,  gene constructs,  gus,  plant promoters,  reporter gene.

19.03.2015, 1834 просмотра.