Monitoring of Сells Weighted Average Size during Cultivation from their Hydrodynamic Orientation

Автор: V.V. Bunin, and V.D. Ivchenko

Страница: 74-79

Monitoring of Сells Weighted Average Size during Cultivation from their Hydrodynamic Orientation

Biotekhnologiya, 2014, N 2, P. 74-79

UDC 57.087.27, 57.083.13

Section:  “Processes and Equipment”

V.V. Bunin ^*,  and  V.D. Ivchenko

The Moscow State Technical University for Radiotechnique, Electronics and Automatics,  119454, Moscow Russia

e-mail:  vadbun@mail.ru

A method for cyclic on-line measurement of weighted average cell size was used for the first time to the monitoring of the Lactobacillus plantarum bacteria culturing. The method is based on the analysis of the cell disorientation in the Tailor-Couette cylindrical flow after its stoppage. The method of microphotographs at varous stages of cell growing was used as reference. The obtained data on the dynamics of the cell size during the lactobacilli cultivation testify to a possibility of the application of the suggested method to the description of cell culturing, its validation and optimization.

Key words:  cells weighted average size monitoring,  hydrodynamic cells orientation.

The full English version of the article was published in “Biotechnology in Russia”, 2014, Issue 2, pp. 74-79 as V.V. Bunin, and V.D. Ivchenko “Monitoring of Сells Weighted Average Size from their Hydrodynamic Orientation during Growth”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=24072640

18.03.2015, 1649 просмотров.