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Pegylated Recombinant Granulocyte Colony-Stimulating Factor with Prolonged Mechanism of Action: a New Scheme for Active Pharmaceutical Substance Production
Pegylated Recombinant Granulocyte Colony-Stimulating Factor with Prolonged Mechanism of Action: a New Scheme for Active Pharmaceutical Substance ProductionАвтор: I.А. Puchkov, D.I. Bairamashvili, I.V. Miagkikh, and V.I. Shvets Страница: 35-50
Pegylated Recombinant Granulocyte Colony-Stimulating Factor with Prolonged Mechanism of Action: a New Scheme for Active Pharmaceutical Substance Production Biotekhnologiya, 2014, N 2, P. 35-50 UDC 577.112.083 Section: “Biologicals Technology”
I.А. Puchkov *, 1, D.I. Bairamashvili 2, I.V. Miagkikh 3, and V.I. Shvets 4 1 The Russian Pharmaceutical Company Masterclone, 119019, Moscow Russia 2 The Shemyakin-and-Ovchinnikov Institute for Bioorganic Chemistry, Russ. Acad. Sci., 117997, Moscow Russia 3 The Russian Innovative Pharmaceutical Company Generium, 123317, Moscow Russia 4 The Lomonosov Moscow University for Fine Chemical Technology, 119571, Moscow Russia e-mail: poutchkov@mail.ru
A production of an active pharmaceutical substance of the PEGylated recombinant granulocyte colony-stimulating factor, G-CSF (Filgrastim), with a prolonged mechanism of action has been carried out in four stages. The first (preliminary) stage included the protein solubilization from inclusion bodies, renaturation and ion exchange chromatography on SP-Sepharose FF at pH 6.0 to obtain a solution with hrG-CSF concentration 12—15 mg/ml. The second stage was performed by the modification of the protein via the selective covalent attachment of α-methyl-PEG-propionaldehyde to α-amino group of the N-terminal methionine in the recombinant G-CSF. The third step consisted of basic purification procedures of PEG-G-CSF by hydrophobic chromatography on Butyl-Sepharose 4 FF at pH 7.0, size-exclusion chromatography on Sephadex G-25 and ion-exchange chromatography on SP-Sepharose FF at pH of 4.5. At the final stage, the obtained preparation (pure according to the European Pharmacopoeia) was transferred to a final buffer by the size-exclusion chromatography on Sephadex G-25. As a result, a technological scheme for the recombinant PEG-G-CSF manufacturing allowing to produce a pharmacopoeia quality preparation with the yield of at least 35% of the initially taken protein was developed.
Kew words: chromatography, Filgrastim, granulocyte colony-stimulating factor, modification, poly(ethylene) glycol.
The full English version of the article was published in “Biotechnology in Russia”, 2014, Issue 2, pp. 35-50 as I.А. Puchkov, D.I. Bairamishvili, I.V. Miagkikh, and V.I. Shvets “Pegylated Recombinant Granulocytic Colony-Stimulating Factor with Prolonged Mechanism of Action: a New Scheme for Active Pharmaceutical Substance Production”. It is contained at the Russian Scientific Electron Library website: http://elibrary.ru/item.asp?id=24072635
18.03.2015, 1669 просмотров. |
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