Russian Journal of Biotechnology Articles archive Archive 2014 N 1 Assessing of Serodiagnostic Potential of Mycobacterium tuberculosis Recombinant Antigens Produced in Various Expression Systems

Assessing of Serodiagnostic Potential of Mycobacterium tuberculosis Recombinant Antigens Produced in Various Expression Systems

Автор: V.I. Dyatlova, A.G. Bogun, and S.F. Biketov

Страница: 72-78

 

Assessing of Serodiagnostic Potential of Mycobacterium tuberculosis Recombinant Antigens Produced in Various Expression Systems

Biotekhnologiya, 2014, N 1, P. 72-78

UDC 616-07:061.62

Section:  “Metrology, Standartization, and Control”

 

V.I. Dyatlova *,  A.G. Bogun,  and  S.F. Biketov

The State Research Center for Applied Microbiology and Biotechnology,  142279, Obolensk, Moskovskaya oblast Russia

e-mail:  varya_dyatlova@mail.ru

 

Six recombinant antigens of M. tuberculosis (Rv0040с, Rv1837c, Rv1860c, Rv2875, Rv2873 and Rv1636-Rv2185с) that were produced by P. pastoris were used in enzyme immunoassay of sera from patients with tuberculosis. The results of the analyses for the first four of these proteins were compared with the data for the same antigens obtained using the E. coli expression system. In all cases, the recombinant proteins produced by P. pastoris proved to be more effective as tuberculosis seromarkers; in particular, their sensitivity was by 17% on average higher. The best ELISA results were obtained with Rv2875 providing the sensitivity of 60% and specificity of 100%.

 

Key words:  antigens,  Pichia pastoris,  serodiagnosis,  tuberculosis.

 

The full English version of the article was published in “Biotechnology in Russia”, 2014, Issue 1, pp. 72-78 as V.I. Dyatlova, A.G. Bogun, and S.F. Biketov “Assessing of Serodiagnostic Potential of Mycobacterium tuberculosis Recombinant Antigens Produced in Various Expression Systems.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=24083408

 

16.03.2015, 1585 просмотров.

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