Characteristics of Biodegradable Micro- and Macro- Cell Carriers based on Recombinant Spidroin

Автор: V.G. Bogush, L.I. Davydova, M.M. Moisenovich, K.V. Sidoruk, A.Yu. Arkhipova, D.G. Kozlov, I.I. Agapov, M.P. Kirpichnikov, and V.G. Debabov

Страница: 52-61

Characteristics of Biodegradable Micro- and Macro- Cell Carriers based on Recombinant Spidroin

Russian Journal of Biotechnology, 2014, N 1, P. 52-61

UDC 577.112.083

Section:  “Utilization of Biopreparations”

V.G. Bogush ^{1, *},  L.I. Davydova ¹,  M.M. Moisenovich ²,  K.V. Sidoruk ¹,  A.Yu. Arkhipova ²,  D.G. Kozlov ¹,  I.I. Agapov ³,  M.P. Kirpichnikov ²,  and  V.G. Debabov ¹

¹  The State Research Institute for Genetics and Selection of Industrial Microorganisms, 117545, Moscow Russia

²  The Faculty of Biology, Lomonosov Moscow State University, 119991, Moscow Russia

³  The Shumakov Research Center for Transplantology and Artificial Organs, 123182, Moscow Russia

e-mail:  bogush@genetika.ru

Gels, microgels and matrices based on a previously designed protein 1F9, a recombinant analog of dragline silk protein (spidroin 1), produced by Saccharomyces cerevisiae strain were prepared and their physical, chemical and biological properties were investigated. It was shown that the microgels obtained from 2.5% protein hydrogel have sizes in the range of 50 —300 microns with a predominance of particles with a diameter of 50 —150 microns. The microgel particles were stable in neutral, acidic and alkaline environments; in corrosive environments (Fenton’s reagent), a 50% (w) degradation of the particles occurred within three weeks. The microgel surface investigated in aqueous medium by confocal laser scanning microscopy and by scanning electron microscopy under dehydration has a complex profile combining nanostructures (250—400) μm in diameter and microstructures (3—7) μm. It was established that either microgel or matrix particles are capable of adhesion by murine fibroblasts 3T3 in vitro and maintaining their growth.

Key words:  adhesion,  cell population,  fibroblasts,  hydrogel,  matrix,  microgel,  proliferation,  spidroin.

16.03.2015, 1784 просмотра.