Influence of Bacillus amyloliquefaciens Bacteria on Growth and Toxin Formation in Fusarium sporotrichioides Fungi

Автор: T.Yu. Gagkaeva, O.P. Gavrilova, A.I. Kuzin, N.I. Kuznetsova, M.A. Nikolaenko, and R.R. Azizbekyan

Страница: 32-37

Influence of Bacillus amyloliquefaciens Bacteria on Growth and Toxin Formation in Fusarium sporotrichioides Fungi

Biotekhnologiya, 2014, N 1, P. 32-37

UDC 632.937:582.231

Section:  “Producers, Biology, Selection, and Gene Engineering”

T.Yu. Gagkaeva ^{1, *},  O.P. Gavrilova ¹,  A.I. Kuzin ²,  N.I. Kuznetsova ²,  M.A. Nikolaenko ²,  and  R.R. Azizbekyan ^{2, *}

¹  The All-Russian Institute of Plant Protection, Russ. Acad. Agric. Sci.,  196608, Pushkin, St.-Petersburg Russia

²  The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

e-mail:  t.gagkaeva@mail.ru,  raziz@genetika.ru

The data on the effect of spore-forming bacteria of Bacillus amyloliquefaciens on growth of Fusarium sporotrichioides fungi in vitro and formation of T-2  toxin are represented. The level of the fungicidal activity of B. amyloliquefaciens was determined from changing of the linear growth rate of F. sporotrichioides. The addition of the bacteria cultural liquid considerably reduced this rate; the lethal dose reducing the F. sporotrichioides growth by 50% (LD₅₀) made up 0,09% of culture liquid. The introduction of the B. amyloliquefaciens cultural liquid in a concentration of 1% provided a 95% decrease in the amount of theT-2 toxin in the medium. However, the effective doses of a chemical reference preparation based on DB-tebuconazol were considerably lower than those of the bacterial culture liquid. Nevertheless, the B. amyloliquefaciens strain is perspective for the design of a  biological agent for the control of the Fusarium-caused plant diseases.

Key words:  Bacillus amyloliquefaciens,  bacteria,  fungi,  fungicidal activity,  Fusarium sporotrichioides,  mycotoxin.

The full English version of the article was published in “Biotechnology in Russia”, 2014, Issue 1, pp. 32-37 as T.Yu. Gagkaeva, O.P. Gavrilova, A.I. Kuzin, N.I. Kuznetsova, M.A. Nikolaenko, and R.R. Azizbekyan “Influence of Bacillus amyloliquefaciens Bacteria on Growth and Toxin Formation in Fusarium sporotrichioides Fungi”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=24083400

16.03.2015, 1971 просмотр .