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Activities of Elements of the Yeast α-Factor Precursor Leader at Different Stages of Somatropin Secretion by Saccharomyces cerevisiae
Activities of Elements of the Yeast α-Factor Precursor Leader at Different Stages of Somatropin Secretion by Saccharomyces cerevisiaeАвтор: K.Yu. Kazachenko, B.D. Efremov, and D.G. Kozlov Страница: 25-31
Activities of Elements of the Yeast α-Factor Precursor Leader at Different Stages of Somatropin Secretion by Saccharomyces cerevisiae Russian Journal of Biotechnology, 2014, N 1, P. 25-31 UDC 582.282.23: 579.6 Section: “Producers, Biology, Selection, and Gene Engineering”
K.Yu. Kazachenko, B.D. Efremov, and D.G. Kozlov * The State Research Institute for Genetics and Selection of Industrial Microorganisms (GosNIIgenetika), 117545, Moscow Russia e-mail: dg_kozlov@genetika.ru
An analytical system for a quantitative assessment of contributions of pre- and pro-components of the yeast α-factor leader to key stages of somatropin secretion in Saccharomyces cerevisiae has been designed. Using this system, it was shown that: 1) the α-factor pro-peptide has contributed almost equally to the secretion at the Erv29p-dependent stage of vesicular transport and at the translocation stage; 2) of the two above stages, the Erv29p-dependent stage could only be optimized by using of two copies of the α-factor pro-peptide; 3) when the α-factor pre-peptide and the artificial pro-peptide with significantly greater hydrophobicity were compared it was found that somatropin secretion apparently depended on the translocation mode as higher secretion was achieved with hydrophobic artificial pre-peptide associated with the co-translational translocation.
Key words: Erv29p, pro-peptide, signal peptide, somatropin secretion, translocation, yeast α-factor leader peptide.
16.03.2015, 1760 просмотров. |
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