Activities of Elements of the Yeast α-Factor Precursor Leader at Different Stages of Somatropin Secretion by Saccharomyces cerevisiae

Автор: K.Yu. Kazachenko, B.D. Efremov, and D.G. Kozlov

Страница: 25-31

Activities of Elements of the Yeast α-Factor Precursor Leader at Different Stages of Somatropin Secretion by Saccharomyces cerevisiae

Russian Journal of Biotechnology, 2014, N 1, P. 25-31

UDC 582.282.23: 579.6

Section:  “Producers, Biology, Selection, and Gene Engineering”

K.Yu. Kazachenko,  B.D. Efremov,  and  D.G. Kozlov ^*

The State Research Institute for Genetics and Selection of Industrial Microorganisms (GosNIIgenetika),  117545, Moscow Russia

e-mail:  dg_kozlov@genetika.ru

An analytical system for a quantitative assessment of contributions of pre- and pro-components of the yeast α-factor leader to key stages of somatropin secretion in Saccharomyces cerevisiae has been designed. Using this system, it was shown that: 1) the α-factor pro-peptide has contributed almost equally to the secretion at the Erv29p-dependent stage of vesicular transport and at the translocation stage; 2) of the two above stages, the Erv29p-dependent stage could only be optimized by using of two copies of the α-factor pro-peptide; 3) when the α-factor pre-peptide and the artificial pro-peptide with significantly greater hydrophobicity were compared it was found that somatropin secretion apparently depended on the translocation mode as higher secretion was achieved with hydrophobic artificial pre-peptide associated with the co-translational translocation.

Key words:  Erv29p,  pro-peptide,  signal peptide,  somatropin secretion,  translocation,  yeast α-factor leader peptide.

16.03.2015, 1796 просмотров.