Adaptation of Rhodococcus rhodochrous M8, a producer of acrylamide, to changes in ammonium concentration in the growth medium

Article: Adaptation ofRhodococcus rhodochrous M8, a producer of acrylamide, to changes in ammonium concentration in the growth medium

O. B. Astaurova, T. E. Leonova, I. N. Polyakova, I. V. Sineokaya, V. K. Gordeev, A. S. Yanenko

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ABSTRACT: The mechanism of adaptation of the acrylamide producing strainRhodococcus rhodochrous M8 to changes in ammonium concentrations in the medium was studied. An increase in the content of ammonium in the medium changed the activity of glutamine synthetase (GS) (EC 6.3.1.2) and glutamine dehydrogenase (GD) (EC 1.4.1.4), the enzymes of ammonium assimilation, as well as the activities of enzymes responsible for nitrile utilization: nitrile hydratase (EC 4.2.1.84) and amidase (EC 3.5.1.4). This also inhibited the activation of GS induced by phosphodiesterase (EC 3.1.4.1 ). Increases in the activities of nitrile hydratase and amidase and resistance of these enzymes to ammonium were observed in mutant ofR. rhodichrous resistant to phosphotricine, an inhibitor of GS. An important role of GS in the mechanism of adaptation is suggested.

07.04.2012, 3679 просмотров.