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Bioengineering: Study results from GosNiigenetika, State Research Institute of Genetics and Selection of Industrial Microorganisms provide new insights into bioengineering
Bioengineering: Study results from GosNiigenetika, State Research Institute of Genetics and Selection of Industrial Microorganisms provide new insights into bioengineeringBioengineering; Study results from State Research Institute of Genetics and Selection of Industrial Microorganisms provide new insights into bioengineering (c) Copyright 2010 Science Letter via NewsRx.com 2010 OCT 19 - (NewsRx.com) -- New research, 'Production of succinic acid at low pH by a recombinant strain of the aerobic yeast Yarrowia lipolytica,' is the subject of a report. "Biotechnological production of weak organic acids such as succinic acid is most economically advantageous when carried out at low pH. Among naturally occurring microorganisms, several bacterial strains are known to produce considerable amounts of succinic acid under anaerobic conditions but they are inefficient in performing the low-pH fermentation due to their physiological properties," scientists writing in the journal Biotechnology & Bioengineering report.
"We have proposed therefore a new strategy for construction of an aerobic eukaryotic producer on the basis of the yeast Yarrowia lipolytica with a deletion in the gene coding one of succinate dehydrogenase subunits. Firstly, an original in vitro mutagenesis-based approach was proposed to construct strains with Ts mutations in the Y. lipolytica SDH1 gene. These mutants were used to optimize the composition of the media for selection of transformants with the deletion in the Y. lipolytica SDH2 gene. Surprisingly, the defects of each succinate dehydrogenase subunit prevented the growth on glucose but the mutant strains grew on glycerol and produced succinate in the presence of the buffering agent CaCO(3). Subsequent selection of the strain with deleted SDH2 gene for increased viability allowed us to obtain a strain capable of accumulating succinate at the level of more than 45?g?L(-1) in shaking flasks with buffering and more than 17?g?L(-1) without buffering," wrote T.V. Yuzbashev and colleagues, State Research Institute of Genetics and Selection of Industrial Microorganisms.
The researchers concluded: "The possible effect of the mutations on the utilization of different substrates and perspectives of constructing an industrial producer is discussed."
Yuzbashev and colleagues published their study in Biotechnology & Bioengineering (Production of succinic acid at low pH by a recombinant strain of the aerobic yeast Yarrowia lipolytica. Biotechnology & Bioengineering, 2010;107(4):673-82).
Additional information can be obtained by contacting T.V. Yuzbashev, Russian State Collection of Industrial Microorganisms, State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-st Dorozhny pr, 1, Moscow, Russia.
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