In vitro and in silico Study of Interferon-Inducing Nucleic Acids Analogs Artificially Programmed to Blockage of HIV Entry into Cells

Автор: A.V. Serbin, A.V. Veselovskii, and V.B. Tsvetkov

Страница: 72-89

In vitro and in silico Study of Interferon-Inducing Nucleic Acids Analogs Artificially Programmed to Blockage of HIV Entry into Cells

Biotekhnologiya, 2012, N 1, P. 72-89

UDC 578.(282+233.3); 544.165; 541.64; 004.942

Section:  “System Analysis, Mathematical Modelling, and Information Systems”

A.V. Serbin ^{1, 2, *},  A.V. Veselovskii ³,  and  V.B. Tsvetkov ^{1, 3}

¹  The Biomodulators & Drugs Research Center, Health Research&Design Fund,  117042, Moscow Russia

²  The Topchiev Institute of Petrochemical Synthesis, Rus. Acad. Sci.,  119991, Moscow Russia

³  The Orerhovich Institute of Biomedical Chemistry, Rus. Acad. Med. Sci.,  119121, Moscow Russia

e-mail:  heal@aha.ru

The approaches to the molecular design of antiviral agents based on the principles of adequacy and mimicry to biopolymers has been discussed. The approaches permit to reproduce pharmaceutically valuable properties of the polymeric basis (backbone), and to create novel non-genetic “programs” for bioactivity of macromolecules by means of grafting specific side group combinations to this backbone. In the in vivo experiments, it was shown that the immunostimulating (in particular, interferon-inducing) antiviral potency of nucleic acids (NA) is characteristic of carboxylic-acid analogs having a NA-like order of furan and anionic components alteration in the polymeric chain. A controlled grafting of virus-targeted side groups to the polymeric basis led to the direct blockage of viral infection in vitro as an additional antiviral effect. In this work, the inhibitors of the early stages of the human immunodeficiency virus type 1 (HIV-1) penetration in cells have been studied. A modeling of the macromolecules interaction with the most probable target, the virus—cell fusion mediator (an α-helical complex of N-region of repeated aminoacid heptades of three HIV-1 envelope glycoprotein gp41 trans-membrane molecules) was performed. The computational docking experiment in silico resulted in the clarification of the putative mechanisms and parameters for programming of site-tropic, axial and belting blockage of the target via the alicyclic type side-groups application. The discovered opportunities of multi-point axial-co-belting blockage of the target opens up new prospects to the prevention/therapy of AIDS, as well as of other viral infections that use similar systems of envelope fusion peptides (of 1^s+ and 3^nd types) for penetration in cells.

Key words:  alternating maleic acid copolymers,  cage alicycles,  docking,  gp41  glycoprotein of HIV envelope,  modeling,  virus—cell fusion inhibitors.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2012, Vol. 48, Issue 9, pp. 723-739 as A. V. Serbin, A. V. Veselovskii, V. B. Tsvetkov “In vitro and in silico investigation of interferonogenic analogues of nucleic acids, artificially programmed to block the initial stages of HIV infection of cells”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683812090049  and  at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=20490337

DOI: 10.1134/S0003683812090049

15.06.2015, 1613 просмотров.