Producing of Enzymatic Hydrolysates from Miscanthus Pulps and their Alcoholic Fermentation

Автор: Ye.A. Skiba, V.V. Budaeva, I.N. Pavlov, Ye.I. Makarova, V.N. Zolotukhin, and G.V. Sakovich

Страница: 42-52

Producing of enzymatic hydrolysates from Miscanthus pulps and their alcoholic fermentation

Biotekhnologiya, 2012, N 6, P. 42-52

UDC 661.725.031.74

Section:  “Biologicals Technology”

Ye.A. Skiba ^*,  V.V. Budaeva,  I.N. Pavlov,  Ye.I. Makarova,  V.N. Zolotukhin,  and  G.V. Sakovich

The Institute for Problems of Chemical and Energetic Technologies, Rus. Acad. Sci., Siberian Branch,  659322, Biysk Russia

e-mail:  eas08988@yandex.ru,  ipcet@mail.ru

Pulps derived from Miscanthus by the nitric-acid or combined hydrolysis have been enzymatically hydrolyzed in acetate buffer under laboratory conditions. The process was primarily scaled up and run in an 11-l fermenter in an aqueous medium. The composition of a multi-enzyme complex comprising BrewZyme BGX, CelloLux-A, and Rapidase CR that provided an effective hydrolysis of the pulps was established and the helpfulness of the simultaneously introduced preparations in an amount of 0,04 g/g of substrate was demonstrated. The dependency of the enzymatic hydrolysis efficiency on the process for Miscanthus pulp preparation was established. The RS concentration in the obtained hydrolysates achieved 70 g/l. The RS in the resultant hydrolysates were converted into ethanol using Saccharomyces сerevisiae Y-1693 strain (All-Russian Collection of Industrial Microorganisms, Moscow). The hydrolysates’ goodness was shown and the economic coefficient of fermentation equal to 0,406 (for the nitric-acid) and 0,443 (for the combined method of pulps preparation), was calculated. The chemical composition of the bioethanol test specimens was examined that provides for a possibility of its further improvement by rectification.

Key words:  acetate buffer,  alcoholic fermentation,  bioethanol,  enzymatic hydrolysis in aqueous medium,  Miscanthus,  pulps,  multi-enzyme complex,  scaling-up.

The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 6, pp. 42-52 as Ye.A. Skiba, V.V. Budaeva, I.N. Pavlov, Ye.I. Makarova, V.N. Zolotukhin, G.V. Sakovich “Producing of enzymaqtic hydrolysates from Miscanthus pulps and their alcoholic fermentation”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=22959636

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