Submerged Heterogeneous Fermentation of Lactic Acid Bacteria

Автор: B.A. Karetkin, N.G. Loiko, I.V. Shakir, and V.I. Panfilov

Страница: 59-68

Submerged Heterogeneous Fermentation of Lactic Acid Bacteria

Biotekhnologiya, 2013, N 1, P. 59-68

UDC 579.864.1: 636.087.8

Section:  “Biologicals Technology”

B.A. Karetkin ^{1, *},  N.G. Loiko ²,  I.V. Shakir ¹,  and  V.I. Panfilov ¹

¹  The Mendeleev University of Chemical Technology,  125047, Moscow Russia

²  The Vinogradskii Institute of Microbiology, Russ. Acad. Sci.,  117312, Moscow Russia

е-mail:  boris.karetkin@gmail.com

A technique of lactic acid bacteria (LAB) submerged heterogeneous culturing in a medium containing Jerusalem artichoke pulp in order to design a probiotic product has been developed. Media composition and fermentation conditions, and also LAB stains were selected. It was shown that the cultivation of LAB in pulp-containing media stimulates the retaining of cells viability during a long-term storage (8 weeks, 5 °C) and enhances their stress tolerance (heating at 50°С for 15 min, freezing—thawing). When bacterial autoregulating agents like alkylhydroxybenzenes were introduced in the LAB biosuspension, the observed effects were enhanced. Taking into account the described techniques, a successful fermentation of Lactobacillus plantarum was performed and laboratory specimens of a product with high LAB content and good biochemical properties were obtained.

Key words:  fructans, inulin,  Jerusalem artichoke,  lactic acid bacteria,  probiotics,  submerged heterogeneous fermentation.

The full English version of the article was published in “Biotechnology in Russia”, 2013, Issue 1, pp. 59-68 as B.A. Karetkin, N.G. Loiko, I.V. Shakir, and V.I. Panfilov “Current Technologies for in vitro Testing of Medicines: Use of Microbioreactors”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21987106

27.04.2015, 1744 просмотра.