Development of Real Time RT-PCR to Identify Bluetongue Serotype 14 Virus

Автор: A.V. Panferova, A.Yu. Kol'tsov, S.Zh. Tsybanov, P.A. Krasochko, and D.V. Kolbasov

Страница: 81-85

Development of Real Time RT-PCR to Identify Bluetongue Serotype 14 Virus

Biotekhnologiya, 2013, N 3, P. 81-85

UDC 619:616.98:578.823.1:577.2

Section:  “Metrology, Standardization, and Control”

A.V. Panferova ^*,  A.Yu. Kol'tsov,  S.Zh. Tsybanov,  P.A. Krasochko,  and  D.V. Kolbasov

The All-Russian Institute for Veterinary Virology and Microbiology, Russ. Acad. Agric. Sci.,  601120, Vladimirskaya oblast, Pokrov Russia

е-mail:  vniivvim@niiv.petush.elcom.ru

an original system oligonucleotide primers—DNA-probe has been designed as a basis for serotype-specific real time RT-PCR to identify bluetongue serotype 14 virus. Data on the assessment of analytical specificity are represented; analytical sensitivity of the method made up 0,75 lg TCD_50/cm³

Key words:  bluetongue virus,  RNA,  RT-PCR real time,  serotype.

The full English version of the article was published in “Biotechnology in Russia”, 2013, Issue 3, pp. 81-85 as A.V. Panferova, A.Yu. Kol'tsov, S.Zh. Tsybanov, P.A. Krasochko, and D.V. Kolbasov “Development of Real Time RT-PCR to Identify Bluetongue Serotype 14 Virus”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=22952563

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