A Bacillus amyloliquefaciens 16-К11 Strain with Fungicidal Activity towards Causes of Grain Crops Fusariosis

Автор: A.I. Kuzin, N.I Kuznetsova, M.A. Nikolaenko, and R.R. Azizbekyan

Страница: 31-39

A Bacillus amyloliquefaciens 16-К11 Strain with Fungicidal Activity towards Causes of Grain Crops Fusariosis

Russian Journal of Biotechnology, 2013, N 5, P. 31-39

UDC 632.937:582.231

Section:  “Producers, Biology, Selection, and Gene Engineering”

A.I. Kuzin ^*,  N.I Kuznetsova,  M.A. Nikolaenko,  and  R.R. Azizbekyan

The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

e-mail:  raziz@genetika.ru

A screening of sporiferous fungicide-producing bacteria has permitted to select a 16-K11 strain that according to the data of the 16S RNA variable segments analysis was identified as Bacillus amyloliquefaciens. Cultural, morphological and physiologobiochemical characteristics of the strain were investigated, and the dynamics of the fungicidal factor(s) synthesis was determined. The location of the fungicidal activity in the culture fluid was confirmed. The fungicidal factor is resistant to proteolytic enzymes. It displays a pronounced activity against the conventional causes of grain crops fusariosis, Fusarium avenaceum, F. graminearum and F. sporotrichioides. The 16-K11 strain CL was shown to be nontoxic for plants.

Key words:  Bacillus amyloliquefaciens,  biological fungicides,  fungicidal activity,  phytopathogenic fungi,  phytotoxicity,  sporiferous bacteria.

01.04.2015, 1664 просмотра.