Study on Conditions for Wolfia arrhiza (L.) Horkel ex Wimm Submerged Culturing in a Modified Bioreactor

Автор: P.A. Khvatkov, M.A. Chernobrovkina, V.V. Sinyov, and S.V. Dolgov

Страница: 51-56

Study on Conditions for Wolfia arrhiza (L.) Horkel ex Wimm Submerged Culturing in a Modified Bioreactor

Russian Journal of Biotechnology, 2013, N 6, P. 51-56

UDC 57.084.1

Section:  “Biologicals Technology”

P.A. Khvatkov ^{1, *},  M.A. Chernobrovkina ¹,  V.V. Sinyov ¹,  and  S.V. Dolgov ^{1, 2}

¹  The All-Russian Research Institute for Agrarian Biotechnology, Russ. Acad. Agric. Sci.,  127550, Moscow Russia

²  The Shemyakin-and-Ovchinnikov Institute for Bioorganic Chemistry, Pushchino Branch, Russ. Acad. Sci.,  142290, Pushchino, Moskovskaya oblast Russia

e-mail:  khvatkov1987@gmail.com

A search for a possibility of Wolffia arrhiza submerged culturing as a promising subject for biofarming has been carried out. Optimum parameters for this process were selected. The highest biomass productivity of the Wolffia population was achieved using a bioreactor and W3M medium under the following cultivation conditions: temperature, 27—29 °C; pH, 5.7—6.0, and photoperiod, 24/0. A modification of the Biostat PBR 2S bioreactor in order to minimize the plant traumatism during the cultivation was performed.

Key words:  biofarming,  bioreactor,  conditions for in vitro culturing,  Wolffia arrhiza.

31.03.2015, 1677 просмотров.