Grounds for the Propionibacterium freudenreichii RVS-4-irf Strain Clinical Trials as a Nutrient Component (nourishment)

Автор: E.P. Ryzhkova, V.V. Serebrov, I.V. Danilova, and T.V. Bykovchenko

Страница: 70-78

Grounds for the Propionibacterium freudenreichii RVS-4-irf Strain Clinical Trials as a Nutrient Component (nourishment)

Biotekhnologiya, 2015, N 4, P. 70-78

UDC 579.676

Section:  “Utilization of Biopreparations”

E.P. Ryzhkova ^{1, *},  V.V. Serebrov ²,  I.V. Danilova ^{1, *},  and  T.V. Bykovchenko ³

¹  The Dept. of Microbiology, Lomonosov Moscow State University (MGU),  119991, Moscow, Russia

²  The State Research Center for Biotechnology Vector,  630559, Kol'tsovo. Novosibirskaya oblast Russia

³  The Research Institute for Bakng Industry, (NIIKP),  107553, Moscow Russia

e-mail:  epr322@mail.ru

The strain of Propionibacterium freudenreichii RVS-4-irf (VKPM B-9654; International  GenBank AC: no. (EU418709) isolated in 1988 from Swiss-type cheese manifests some probiotic-significant features having appropriate effects on animal organism (quails). Its cells demonstrate rather high level of survival under aggressive conditions of a model system of GIT, about 60% log CFU/ml. Unlike lacticacid bacteria, P. freudenreichii has low growth requirements, which is accompanied by the production of multiple nutraceuticals: vitamins of the B group, bifidogenic factor, essential amino acids and others. In view of the above said and the previously shown pronounced antioxidant activity, the investigated strain is of interest as a transient probiotic with the putative in vitro activity. The P. freudenreichii strain is capable of producing a high level of vitamin B₁₂ and propionates, demonstrated proteolysis at neutral pH-values and a spectrum of antibacterial activity and immunotropic effects determined from the cytokine biosynthesis stimulation (shown ex vivo). As a whole, the origin, properties and effects of the strain allows us to recommend it for trials in human clinic nutrition.

Key words:  antibacterial spectrum,  cytokines,  immunomodulation,  model GIT,  probiotic effect,  probiotic potential,  propionates,  Propionibacterium freudenreichii RVS-4-irf,  proteolytic activity,  survival,  vitamin B_12.

06.11.2015, 1421 просмотр .